High Content Screening (HCS) uses microscopic imaging to observe, measure and analyse multiple parameters within cells in a biological assay.
By using fluorescent dyes, fluorescently labelled proteins and fluorescently labelled antibodies, events that occur within cells following the addition of compounds and ligands can be detected and quantitated.
In addition to our in-house CellInsight CX5 platform, our scientists have access to the Image Xpress Ultra from the Molecular Devices Corporation. Examples of the types of assays we can perform using these instruments are shown below
We have a dedicated image analysis service offering custom algorithm generation or the use of standard algorithms to analyse and quantify your assay.
With proprietary autofocus and integrated plate scanning intelligence methods, the CellInsight CX5 HCS Platform brings speed and accuracy to investigate cell populations and phenotypes without sacrificing sensitivity and resolution. The CX5 uses five channel fluorescent imaging allowing analysis of single cells in up to five fluorescent colours. The CX5 is compatible with 96, 384 and 1536 well plates.
The Ultra is a true point scanning confocal microscope and can capture higher resolution images than the Micro. It houses four solid state lasers with an excitation range of 405 and 633 nm.
The Ultra has 10x, 20x, 40x and 60x air objectives and can image most well plate sizes. The Ultra is served by a plate loading robot that, when combined with automated analysis procedures gives us close to high throughput screening capabilities.