
A comprehensive service including:


Our “fee for service” model is simple; we charge a set fee for our assay development services and a subsequent fee per well for each compound screened. A typical service has the following description and deliverables:
If the assay is built for high throughput screening, we would perform an assay robustness step.
Assay Development and Pharmacological Validation
Service
Assay Development and Pharmacological Validation
Description
Using recombinant cell line, primary cells or protein to develop a fit for purpose assay
Deliverables
Assay built for either iterative screening or HTS purposes (e.g. FLIPR, protease, kinase, label free) using cell line or protein provided by client or commercially sourced.Assay validated using pharmacological characterisation of up to 8 standard compounds to XC50
Price
POA
Assay Robustness testing
Service
Assay Robustness for HTS purposes
Description
Assay development performed by Aurelia Bioscience or client assay transferred to Aurelia Bioscience – little or no assay development required
Deliverables
Correlation of a set of compounds and pharmacology of standards between replicates within and between days or comparison of pharmacology were necessary between client data and Aurelia Bioscience data together with assessment of assay quality using Z-factors and reproducibility
Price
POA
The screening of large numbers of compounds will also introduce savings in terms of the fee per well as we can pass on bulk discounts on externally sourced reagents to our clients.
Screening
Service
Cell based OR Biochemical based Screening (HTS)
Description
10,000 to 500,000 compounds*
* Note: Compound numbers are used to exemplify our capability and throughput
Deliverables
In all cases below we would perform the primary screen (one compound per well) provided by the client either in assay ready plates or as a transfer from a source “mother” plate provided by the client and transferred into the assay. Retest would be in triplicate at the same concentration after analysis and feedback from the client on compounds to re-screen. These would be “cherry-picked” and re-screened. XC50 data would be generated using 10 point dose- response curve as directed by the client using the data generated in the retest phase to select the most appropriate compounds for XC50 determination
Price
POA
We have two examples shown here, involving either one technology and a number of targets or one target and a number of technologies. In each case, following the development of the assay, the pharmacological characterisation of the clients compounds would be performed.
Exploratory Biology
Service
Exploratory Biology: Example 1
Description
One technology used to evaluate the activity of three targets
Deliverables
Evaluating up to three cell lines or reagents(proteins) in the same technology format e.g. three targets, each cell line or protein tested in label free with pharmacological characterisation up to 4 standard compounds to XC50
Price
POA
Exploratory Biology: Example 2
Description
One target evaluated using up to three technologies
Deliverables
Evaluating one cell line or protein in a number of biological readouts e.g. for cell based GPCR receptor coupled through Gi, technology may included FLIPR together with label free response and cAMP measurement on same target to pharmacologically characterise the responses using up to 4 standard compounds (XC50)
Price
POA
Consultancy
In addition we also provide a consultation service on the development of assay cascades designed to increase the physiological relevance of your data whilst reducing the number of non-specific compounds in the output by developing the most appropriate assays for the project.

50,000 compounds screened through cell-based assay to IC50 then selectivity cell assay against related receptor.
Client’s aim:
- Identify compounds that interacted (either in a stimulatory or inhibitory manner) with a two pore non-voltage gated potassium channel implicated in migraine
- Identify hit compounds from a 50,000 compound collection
- Retest and IC50 hits to identify active compounds (inhibitors of activators of the channel)
- Perform a selectivity screen with the active compounds against another two pore channel expressed in the heart – remove compounds with activity against this channel
- Client had a less than a six month timeline to complete all studies
Aurelia Bioscience’s role:

