We specialise in physiologically relevant assays using cell-based formats in state of the art technologies i.e. phenotypic screening.
We have used a number of human primary cells in different assay formats. Example:
- Measurement of calcium mobilisation from neutrophils following agonist addition in the presence or absence of compounds using FLIPR in 384 well and 1536 well format.
- Measurement of superoxide burst from cells stimulated with and in the presence of compounds using chemiluminescence substrates.
- Measurement of activation of cells via surface measurement changes and the dissection of G-protein coupled events detected using label-free technology and as a replacement to whole blood CD11b assays.
Fig. Screening of Compounds in Human Neutrophil FLIPR antagonist assay in 1536 well plates. Human neutrophils were isolated from the whole blood of donors by densite gradient centrifugation, loaded with Fluo-loading dye, dispensed into 1536 well plates incubated in the presence of compounds, and screened with agonist addition on-line. Responses from selection of wells are shown in the image.