With over 50 years of experience, we are adept at all aspects of cell culture
We have developed methodologies to grow, sub-culture, harvest, cryopreserve and revive these quantities of cells from single batches, thus reducing screening variability, allowing cells to be used in an identical manner to other assay reagents, reviving and using what is required on a daily basis. Typical screens have required up to 1010 cells.
Our experience includes:
working with CHO, HEK-293, Hela, SHSY5Y adherent cells in addition to non-adherent cells
sub-culturing cells for colony selection from a single 6 well plate through small flasks up to 10 layer cell factories
harvesting and cryopreserving large preparations of cells, as many as 5×10¹º in a single batch
plating and growing cells in 96, 384 and 1536 well plates using various liquid handling approaches
We can support your project by:
developing a cell line for your target and screening application
selecting the most appropriate clone for screening purposes
manufacturing enough cells for your screening needs
screening your target in our labs using your cell line