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Western Assays on ‘Wes’
Whilst Western blotting remains the pre-eminent method of choice for protein detection and characterisation, the various improvements to individual aspects of Western work flow developed in recent years have had little impact on the relatively poor reproducibility, lack of accurate quantification, extensive time to result and reliability issues associated with traditional Western blotting.
The WesTM system from ProteinSimple is a fully automated device which runs the Simple Western size assay; a highly reproducible and sensitive alternative to standard Western blotting assays. Wes employs capillary electrophoresis combined with chemi-luminescence based antibody detection to run the entire Western immunoassay process from start to finish, providing dramatic improvements in sensitivity, reproducibility, quantitative accuracy and time to result compared to all other legacy Western blotting methods. Simple Western assays cover three separation ranges: 2 – 40kDa, 12 – 230 kDa and 66 – 440kDa.
In addition to Simple Western Immunoassay, Wes also runs the Simple Western Total Protein assay
Watch the video below to learn more about WES:
Total protein and immunoassay detection of decreasing concentrations of DNAK in HeLa lysate (15, 7.5 and 3.75 μg/mL in the Total Protein Assay on the left, 0.015, 0.0075 and 0.00375 μg/mL in the immunoassay on the right).