How it works
- Aurelia Bioscience uses Ultra Low Attachment (ULA) plates to generate and culture spheroids. These plates have an ultra-low attachment surface coating and a well geometry that favours the formation of a single spheroid within each well
- Spheroids of different sizes develop gradients of oxygen, nutrients and metabolites, creating a hypoxic core and replicating cells on the outer edges
- Drug potency and efficacy is monitored in either an imaging-based assay (on a wide-field imaging plate reader) or by using commercially available assays that track parameters such as cell viability
Fig. Spheroids formed from U87 cells then treated with Paclitaxel. The spheroid decreases in size upon treatment and incubation with compound over 48 hours in culture.
Some of the advantages of this technology are:
We have grown numerous cells in a 3D format
We have treated the spheroids with various drug treatments and have monitored their response using Draq 7 (which only penetrates dead cells), Calcein AM (which only penetrates live cells) and Hoeshct for an overall cell count. We have also used to CellTire Glo to determine viability by looking at ATP levels.
How it works
- Aurelia Bioscience has developed a 3-D micro-tissue system from electrospun material that can be used in conjunction with well plates for higher throughput screening
- Electrospun material mimics the natural extracellular matrix and provides an ideal substrate for cells to adhere to
- We have re-engineered electrospun material to form micro-scaffold islands on to which we seed, grow and differentiate cells prior to performing more conventional assays in well plates. Cells grow on, around and into the material, forming a micro-island of adherent cells that are effectively “micro-tissues in solution”
- The incorporation of iron particles into fibres during manufacture results in scaffolds that can be physically manipulated using magnetism